慢病毒表达载体pLV-EF1-dCas9-KRAB-T2A-BSD-自主发布-资讯-生物在线

慢病毒表达载体pLV-EF1-dCas9-KRAB-T2A-BSD

作者:北京百奥创新科技有限公司 2023-03-28T16:32 (访问量:6451)

Description:CRISPR (clustered regularly interspaced short palindromic repeats) interference (CRISPRi) is a widely used genetic technique that allows targeted silencing of transcription in bacteria and mammalian cells. The CRISPRi system is comprised of a catalytically inactive Cas9 (dCas9) protein fused with a transcriptional repressor such as the Krüppel-associated box (KRAB) repressor and a customizable single guide RNA (sgRNA). The Cas9-sgRNA complex binds to DNA elements complementary to the sgRNA and causes a steric block that halts transcript elongation by RNA polymerase, resulting in the repression of the target gene.

pLV-EF1-dCas9-KRAB-T2A-BSD Lentiviral Vector expresses the dCas9-KRAB fusion with EF1 promoter and allows for BSD selection of transduced cells.

Vector InformationThis is a lentiviral expression vector that contains all the elements necessary for efficient and high yield viral production. A ubiquitous EF1 promoter drives the expression of the dCas9-KRAB fusion protein and BSD to allow for selection of transduced cells. This vector can be used for stable cell line generation or in combination with a gene specifc sgRNA to repress the target gene expression.

IMPORTANT NOTICEStore the vial at -20°C immediately upon receipt.


Note: Bacterial culture of pLenti vectors should be done in medium containing 10 μg/mL Ampicillin. For maximal plasmid yield and quality, we recommend Stbl3 competent cells (Invitrogen).
Specifications:
Product NamepLV-EF1-dCas9-KRAB-T2A-BSD
Shipping Condition

Room Temperature - 2 Day Shipping

Storage and Stability

Store at -20°C immediately upon receipt. This product is stable for 6 months when stored as directed.

Quality Control

This plasmid is sequence verified.

Restricted Use

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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