- Product name
EliKine™ Human IL-12 p70 ELISA Kit
- Reactivity
Human
- Applications
ELISA
- Application notes
EliKine™ Human IL-12 p70 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-12 in samples. An antibody specific for IL-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-12 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
- Detection method
Colorimetric
- Sample type
Cell culture supernatants, Other biological fluids, Plasma, Serum
- Assay type
Sandwich ELISA (quantitative)
- Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Fig.1. Human IL-12 p70 Standard Curve.
Fig.2. EliKine™ Human IL-12 p70 ELISA Kit is a sandwich ELISA to quantitate IL12 in samples.
Product Properties
- Kit components
• Human IL-12 p70 microplate
• Human IL-12 p70 standard
• Human IL-12 p70 detect antibody
• EliKine™ Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers. - Features & Benefits
EliKine™ Human IL-12 p70 ELISA Kit has high sensitivity and excellent specificity for detection of Human IL-12 p70. No significant cross-reactivity or interference between Human IL-12 p70 and analogues was observed.
- Calibration range
7.8 pg/mL-500 pg/mL
- Limit of detection
4 pg/mL
- Usage notes
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate. - Storage instructions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
- Shipping
Gel pack with blue ice.
- Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Additional Information
- Background
IL12A gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
- Gene ID
- Alternative names
IL12; IL 12; IL-12; CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain
- Accession