PDK2 (Human) Recombinant Protein
产品名称： PDK2 (Human) Recombinant Protein
英文名称： PDK2 (Human) Recombinant Protein
产品编号： P5616
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
- 联系人 :
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- 邮编 : 11493
- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Human PDK2 (NP_002602.2, 1 a.a. - 407 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
- Theoretical MW (kDa):
- 73
- Form:
- Liquid
- Preparation Method:
- Baculovirus infected insect cell (Sf21) expression system
- Purification:
- Glutathione sepharose chromatography
- Purity:
- 77 % by SDS-PAGE/CBB staining
- Activity:
- The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: PDHKtide. ATP: 100 uM.
- Storage Buffer:
- In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
- Storage Instruction:
- Store at -80°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Loading 1 ug protein in SDS-PAGE
- Note:
- Result of activity analysis
-
- Application Image
- Functional Study
- SDS-PAGE
- Entrez GeneID:
- 5164
- Protein Accession#:
- NP_002602.2
- Gene Name:
- PDK2
- Gene Alias:
- PDHK2
- Gene Description:
- pyruvate dehydrogenase kinase, isozyme 2
- Omim ID:
- 602525
- Gene Ontology:
- Hyperlink
- Other Designations:
- pyruvate dehydrogenase kinase, isoenzyme 2