XAF1 polyclonal antibody
产品名称： XAF1 polyclonal antibody
英文名称： XAF1 polyclonal antibody
产品编号： PAB12307
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of XAF1.
- Immunogen:
- A synthetic peptide corresponding to amino acids at C-terminus of human XAF1.
- Sequence:
- QEKCRWLASSKGKQVRNFS
- Host:
- Rabbit
- Theoretical MW (kDa):
- 34.6
- Reactivity:
- Human, Mouse, Rat
- Specificity:
- Identical to the related rat and mouse sequence.
- Form:
- Lyophilized
- Purification:
- Affinity purification
- Storage Buffer:
- Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
- Storage Instruction:
- Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Identification of XAF1 as an antagonist of XIAP anti-Caspase activity.
Liston P, Fong WG, Kelly NL, Toji S, Miyazaki T, Conte D, Tamai K, Craig CG, McBurney MW, Korneluk RG.Nat Cell Biol. 2001 Feb;3(2):128-33.
- 2.
- Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines.
Fong WG, Liston P, Rajcan-Separovic E, St Jean M, Craig C, Korneluk RG.Genomics. 2000 Nov 15;70(1):113-22.
- Applications
- Western Blot
- Western Blot analysis of XAF1 expression from cell and tissue extracts with XAF1 polyclonal antibody (Cat # PAB12307).
Lane 1 : rat liver tissue lysate.
Lane 2 : rat kidney tissue lysate.
Lane 3 : rat spleen tissue lysate.
Lane 4 : human placenta tissue lysate.
Lane 5 : Marker.
Lane 6 : MCF-7 whole cell lysate.
Lane 7 : HeLa whole cell lysate.
Lane 8 : smmc cell lysate.
Lane 9 : HT1080 whole cell lysate.
Lane 10 : Colo 320 whole cell lysate.
- Entrez GeneID:
- 54739
- Gene Name:
- XAF1
- Gene Alias:
- BIRC4BP,HSXIAPAF1,XIAPAF1
- Gene Description:
- XIAP associated factor 1
- Omim ID:
- 606717
- Gene Ontology:
- Hyperlink
- Gene Summary:
- X-linked inhibitor of apoptosis (XIAP; MIM 300079) is a potent member of the IAP family. All members of this family possess baculoviral IAP (BIR) repeats, cysteine-rich domains of approximately 80 amino acids that bind and inhibit caspases (e.g., CASP3; MIM 600636). XIAP has 3 BIR domains and a C-terminal RING zinc finger that possesses E3 ubiquitin ligase (see MIM 601623) activity. XAF1 antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells (Liston et al., 2001 [PubMed 11175744]).[supplied by OMIM
- Other Designations:
- BIRC4 binding protein